Device

Part:BBa_K1377000:Design

Designed by: Jade Minseob Yoo   Group: iGEM14_Korea_U_Seoul   (2014-10-09)


promoter of pili A gene cluster + spaA front


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 109
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 109
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 838
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 109
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 109
    Illegal NgoMIV site found at 439
    Illegal NgoMIV site found at 1091
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 572


Design Notes

Just include all cluster that related to make pili A

And there is no good restriction enzyme unique cut site, so we slice the cluster to two

Source

Genomic DNA of Corynebacterium diphtheriae

References